|
KMID : 0385520100230020172
|
|
Analytical Science & Technology
2010 Volume.23 No. 2 p.172 ~ p.178
|
|
|
Study on the immobilization of plant glutathione S-transferase for development of herbicide detection kit
|
|
Cho Hyun-Young
Lee Jin-Joo
Kong Kwang-Hoon
|
|
|
Abstract
|
|
|
|
Glutathione S-transferase is known to play a crucial role in detoxification in many cases. To develop a herbicide detection biosensor, we in this study attempted to immobilize glutathione S-transferase enzyme on solid supports, polystyrene and agarose, and Na-alginate. These matrixes were attractive materials for the construction of biosensors and might also have utility for the production of immobilized enzyme bioreactors. We also compared the activities of glutathione-S-transferase immobilized OsGSTF3 and free OsGSTF3. The specific activity of the free enzyme in solution was 3.3 higher than the immobilized enzyme. These results suggest that 50% of the enzyme was bound with the catalytic site in polystyrene-alkylamine bead and immobilized enzymes showed 80% remaining activity until 3 times reuse.
|
|
|
KEYWORD
|
|
|
biosensor, glutathione S-transferase, herbicide detoxification, protein immobilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|